Goals from Aaron Sennett-Neilson and Javanne Smith helped the youth team to a 2-1 win over Scunthorpe United at St George's Park, Sheffield Graves yesterday.
Sennett-Neilson opened the scoring in the first half, with a fine effort, before the visitors equalised during the second period.
However, the Spireites recorded back to back wins in the EFL Youth Alliance North East thanks to Smith's header.
The youth team are next in action when they travel to York City on Tuesday, 11am, in what is their final match of the season.
Chesterfield XI vs Scunthorpe: Marris; Howard, Sharman, Willock, Render; Ofoegbu, Sennett-Neilson, Hand; Russ, Foster, Smith.
Substitutes: Walker, Turner, Wild, Emery, Gibson.