The youth team suffered a second consecutive defeat yesterday, as Mansfield Town came from 1-0 down to win 2-1 at St George's Park, Sheffield Graves.
Ify Ofoegebu gave the Spireites the lead in the first half, when after he latched on to Levi Amantchi's pass, he showed good composure before striking the ball into the bottom left corner of the net.
However, the visitors hit back twice in the closing stages of the game, with both goals coming from set-pieces.
Many of the Spireites' squad are set to be involved in Tuesday night's Derbyshire FA Senior Challenge Cup final at Alfreton Town, 7.45pm.
Chesterfield XI vs Mansfield: Marris, Howard, Hand, Render, Feurtado, Emery, Ofoegbu, Holmes, Sennett-Neilson, Walker, Amantchi.
Substitutes: Rawson, Foster, Turner, Smith, Wild.