The Spireites went down to a 2-1 defeat at home to Accrington Stanley on Saturday - a result which led to the departure of manager Gary Caldwell.
Kristian Dennis equalised from the penalty spot midway through the second half after Billy Kee opened the scoring, but substitute Mallik Wilks struck the winner for Accrington late on. Chesterfield also had Bradley Barry sent-off.
Caldwell made two changes to the starting line-up for what proved to be his final game in charge. Joe Anyon replaced the injured Tommy Lee in goal while Andy Kellett came in at the expense of Matthew Briggs, who was on the substitutes bench.
Anyon made a good save early on to keep out Sean McConville's effort.
Kellett then picked out Kristian Dennis inside the area, but Farrend Rawson and Mark Hughes combined to block his route to goal.
Kee had a shot charged down before Jordan Flores had Chesterfield's first chance of the game, flicking a header wide.
Jordan Clark put a shot on target but it lacked the power to test Anyon.
Anyon was forced to touch Kee's header onto the bar and then tipped over Sean McConville's long-range effort.
The Spireites threatened early in the second half with Sam Hird having a shot blocked and Flores seeing his follow-up deflected over.
Dennis also had an effort well-saved by former Chesterfield keeper Aaron Chapman.
Chesterfield had a strong appeal for a penalty turned down when Janoi Donacien appeared to handle the ball inside the area from Barry's cross.
Robbie Weir then saw his effort from distance went just over.
Chesterfield were reduced to ten men in the 63rd minute when Barry was shown the red card following a late challenge on Seamus Conneely.
Kayden Jackson had a shot blocked by Hird inside the area before Accrington took the lead.
McConville's cross from the left was brought down by Kee (68) at the far post and he fired past Anyon.
McConville then put a free-kick over and Jackson saw his long-range effort go off-target.
The Spireites pulled a goal back when Dennis (77) scored from the spot after being fouled inside the area.
Accrington applied pressure with Tyler Forbes seeing his shot turned wide by Anyon and Clark headed straight at the keeper.
Wilks (82) scored the winner, curling a low shot beyond Anyon from the edge of the area.
Wilks went close to a third for the visitors when his shot was saved by Anyon with his legs.
Chesterfield: Anyon, Hird, Wiseman, Barry, Jules (Ugwu 89), Kellett (Sinnott 73), Flores, Weir (O'Grady 73), Reed, De Girolamo, Dennis
Unused substitutes: Jones, Maguire, McCourt, Briggs
Accrington Stanley: Chapman, Hughes, Donacien, Nolan, Clark, Jackson (Wilks 77), McConville, Thorniley (Forbes 67), Rawson, Conneely, Kee
Unused substitutes: Brown, McLeod, Sykes, Johnson, Savin
Attendance: 4,487 (198 away fans)