The Spireites will face Rotherham United at home and take on three local non-league teams as part of their pre-season programme.
Rotherham, who were relegated from the Championship at the end of last season, will visit the Proact on Tuesday, July 25 (7.30pm). Admission will cost £10 for adults, £5 for concessions and £3 for juniors.
Before then, Chesterfield will face Sheffield FC at the Coach & Horses ground on Tuesday, July 4 (7.30pm).
They will make the trip to Matlock Town on Thursday, July 6 (7.30pm) and then contest the G F Tomlinson Group Cup at Buxton (7.30pm) the following night. Admission to the Matlock game will be charged at £10 (adults), £5.50 (senior citizens) and £2 (juniors).
Admission details for the matches at Sheffield and Buxton will be announced later.
A game at home to Sheffield United on Tuesday, July 18 (7.30pm) was announced earlier this month.