Spireites fans have moved to the upper tier of the South Stand for Saturday's game at Bolton Wanderers.
This change has been made due to the huge demand for tickets from home fans, who are now being sold tickets within the South Stand lower tier.
Although tickets for Chesterfield fans say Block E on them, entry will be via Block F in the upper tier. All seats will be unreserved.
This will not affect disabled supporters, who can enter via the South West Tunnel.