Sadiq El Fitouri is facing disciplinary action after failing to report for duty.
The defender has not been in contact with club officials since last Wednesday, after being given Thursday off to attend to a personal matter.
Director & company secretary Ashley Carson said: "The player will be fined in accordance with our code of conduct and we will be writing to him to confirm our position. We will then reserve our right to take further action if deemed appropriate.”
El Fitouri has made two appearances since joining the Spireites at the beginning of February. He was a free agent after being released by Manchester United.