The Spireites' reserve side get their season under way tomorrow afternoon as they face Grimsby Town away from home.
The side came within touching distance of winning the league last season, pipped to the post by Notts County, who the Spireites also played a recent warm-up match with.
The match kicks-off at the Mariners' Blundell Park ground at 2pm, with admission costing £3 for adults and £2 for juniors.
Player-coach Ritchie Humphreys will again lead the side this season, and he gave his view of the away starter: "With the reserve team it can chop and change for any given week and on Tuesday we've got quite a young team going to Grimsby.
"This is brilliant because we've got some younger professionals who are trying to get their way into the first-team squad, we've got some first and second year scholars who are pushing and who I've been impressed with so far when they've come to training with us and in pre-season friendlies.
"We've had our warm-up game as a group and the league starts now. The important thing for me is that the players take on board all that the manager has said to them in pre-season and this younger group take that on board and show us that they're willing, eager and ready to take on information.
"Regardless of the result, I want to see that implemented tomorrow."
Humphreys himself won't be playing in Lincolnshire, but is looking forward to the challenge faced from the dugout: "I'm excited, taking a team is very much different from helping out or even playing in a team and coached from the middle of the pitch. From Tuesday's point of view I'll be on the sidelines taking the team, giving them information that are the principles that run through the football club.
"We came close to winning the league last year, but the ultimate goal is to get some of these players into our first team."