Right-back Paul McGinn joined a busy treatment room at the weekend after limping off with a hamstring injury during the second half away at Bury.
Starting with the situation at full-back, the manager said: “We don't know the level of Paul McGinn’s injury just yet, it could be anything from two to six weeks. At the moment we aren't sure, we will just keep our fingers crossed and wait and see.
“Dion Donohue is having a scan today (Monday) as well so we will find out the details of that later in the afternoon today.
“We are going to have to reshuffle the pack again and look at one or two of the younger lads in the squad as to whether or not they will come to the plate, but they will certainly be in contention.
“We will see tomorrow when we have got all the facts in front of us and know who is going to be available.”
Striker Ched Evans was another that missed the match at Bury and Wilson admitted that he was hoping the Welshman would have been available for the tie, he continued: “Evans was one we were hoping would be fit for the weekend, but it wasn’t worth gambling and it won't be worth gambling again tomorrow if he isn't close enough.
“We have to be looking at the long term as opposed to getting him back for one game, hoping he scores and then losing him again for another six games. We will assess him tomorrow but will be very careful with him.”
The changes to the loan system this season have seen some teams facing injury problems, none more so than the Spireites, but Wilson insists that these changes are positive as it gives the younger players a chance to come into the fold.
“There are currently nine first team players that are out and we can’t afford two of them, let alone nine.
“Whether one of those will be fit for tomorrow or the weekend we will wait and see but a lot of teams have got the same problem, just maybe not to the same extent as us at this moment.
“It is an opportunity for one of the young kids and if it is a case that one is named in the squad, whoever that may be, then we will wish him all the best.
“Hopefully he can then stay in the team and add more competition, because you never know, one or two of these boys may surprise a few people and we are hoping that that will be the case.”