The Spireites line-up against the Blades in front of the TV cameras on Sunday afternoon, with points as well as local pride at stake at the Proact.
A bumper crowd is expected in North Derbyshire for the game, as the Blues look to get back to winning ways in the league against the side from just across the county line.
Due to police advice, there will be no tickets available on matchday, with all tickets for the match needing to have been bought by 6pm on Friday evening.
Sam Hird continues on the sidelines for the Spireites, with manager Danny Wilson confirming that the centre-back faces another two weeks or so out with a hernia injury.
The Spireites otherwise have no new injury concerns, though they are also not expecting any of the currently unfit players back for the Blades test.
Former Spireites loanee Leon Best is in contention after he made a Checkatrade Trophy return in midweek and is joined on the injury comeback by fellow attacker Matty Done.
Another former Proact man, Caolan Lavery is also pushing for a place in the side after making a first Blades start in the EFL Trophy game with Grimsby.
The Spireites haven't won any points in the past seven days, but two morale-boosting cup victories away at Colchester United and Crewe Alexandra have lifted the mood at the Proact Stadium.
However, Chesterfield still find themselves in a perilous position in the league table - sitting in 23rd place and without a league win since The mid-September win over Northampton Town.
In opposition, Sheffield United also completed a cup double-header in the past week and in Sky Bet League 1 are the form side in the division, 11 League One games without defeat, with a full eight of those games ending in Blades wins.
The run sees United on an upward trajectory, currently fourth in the table under Chris Wilder's stewardship, six points below runaway leaders Scunthorpe United.
From the Dugout
Wilson spoke before the game about the contest against one of his former sides, recognising the importance of the afternoon to the Proact faithful.
"It's a big one for the fans," he said. "It's a derby game so they will be looking forward to it. We look at it as a big, big challenge because we know they're going to be challenging at the end of the season along with two of three other teams.
"It's going to be a good occasion for the fans, but more importantly we need to maintain the belief that we can get three points off of them."
Much of the build-up has focused on former Sheffield United and current Chesterfield striker Ched Evans, who comes up against the Blades for the first time since leaving the club. However, his manager says the striker has prepared just as for any other game.
"Ched's been no different at all, he's been no different whatsoever," Wilson explained."He was a bit gutted he didn't play the other night as he wanted to play and keep on with his goal-scoring exploits.
"He was on the score sheet last week at Colchester and that's what goal scorers do, they want to get the next one - and that's what Jay O'Shea did.
"He's trained exactly the same and his build up has been no different at all. I'd be surprised if he didn't get a good reception, but I don't think he's to bothered about that, it's more important from his point of view that he puts in a good shift for us.
"He's been playing very well for us - though he did miss a spell with a heel injury - but he's come back and I'm sure he'll want to put in a very good performance and pull us away from the bottom of the league."
The Proact may well be busy on Sunday, but even if you're not packed in yourself you can follow all the action. We have live commentary on PlayerHD, minute-by-minute coverage on Twitter, as well as updates and images on Facebook and Instagram.