The Spireites bring their Checkatrade Trophy group to an end at Crewe Alexandra, with their fate in their hands and qualification on the line.
All four teams in the group sit level on three points going into the final fixtures and a win - or a draw followed by a penalty shoot-out victory - would send Chesterfield into the knock-out stages.
There were no advance tickets made available for this match, with away fans able to pay on the day at Gresty Road. Further details here
As per competition rules, the Spireites must field five players who played in the previous or following league game, and with a squad still facing a number of injuries a strong squad is expected.
Sam Hird misses the game, as he did the FA Cup clash at Colchester United, with an injury and Danny Wilson is unlikely to risk players not fully fit.
With a packed schedule of his own to navigate, Alex boss Steve Davis has said he will rotate his squad - with Ollie Turton and Chris Dagnall in line for rests after picking up injuries at the weekend.
The Spireites brought their goal drought to an end at the weekend with their 2-1 win in the FA Cup and will be hungry for more as they attempt to seal their qualification to the knock-out stage with a win at Gresty Road.
A point coupled with a penalty shoot-out victory (which would take place straight after the 90 minutes) would also be enough for the Spireites to make the second round.
Crewe currently sit in the League Two play-off places, but have won just one of their last nine matches, collecting five draws in that same spell, and made the draw for the second round of the Cup after a 1-1 draw away at Cheltenham on Saturday.
From The Dugout
Speaking after the game at Colchester, Wilson said he would be assessing a number of his players who needed treatment during the Cup win, a list which included Liam O'Neil, Ched Evans, Dion Donohue and Connor Dimaio.
"They're looking okay," said the manager. "The kicks and the bumps and the bruises don't seem half as bad when you've won. I'm hoping they'll be okay and we can get everyone rallied together ahead of the game on Tuesday."
"It's going to be a mixture, it has to be a mixture, because we have to play at least five of the players from the last game. It depends who's fit and who we have available.
"We'll see who's fit, we'll see who's available and we'll carry on from there."
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