The Spireites may have had their unbeaten streak snapped last time out, but they can win their third straight away game with three points at Glanford Park.
Standing in the way are an in-form Scunthorpe United team with half an eye on the play-offs - and not beaten at home in the league by Chesterfield in over a quarter of a century.
Approximately 450 Spireites have purchased tickets for the game in advance, with away fans also able to purchase tickets on the day at Glanford Park.
Liam O'Neil returns to the fold after missing last weekend's Walsall match with illness and defender Charlie Raglan is also available for selection after two recent appearances in the reserve team.
Dion Donohue misses out through suspension after his straight red card last Saturday, the 22-year-old set to miss the next three Spireites matches as a result.
The Iron are without Sean McAllister after the midfielder picked up an ankle injury in training this week, while Scott Wiseman misses Saturday and the rest of the campaign due to upcoming surgery on a bone growth.
However, captain Stephen Dawson is back in training and hoping to be ready for the visit of the Spireites.
Saturday's visitors will be looking to regroup after their short-handed loss to Walsall last Saturday, a game in which the Spireites took the lead before losing goals and Donohue in a 4-1 loss.
Four points above Oldham Athletic in the final relegation spot, Spireites boss Danny Wilson will be well-aware that his side could get sucked quickly back into the mire without a steady stream of incoming points.
The Iron have been on an 11-game tear featuring just one loss since parting company with Mark Robins in mid-January, caretaker Nick Daws has, however, seen the wins become draws in recent times, with United drawing four of their last five games.
The good form has pulled the Iron well clear of the relegation dogfight, and seven off the top six in Sky Bet League 1.
From the Dugout
Ahead of the match manger Wilson spoke to PlayerHD about the match and his side's plan to grab all three points.
He said: "They're in an unbeaten streak and they'll think they can still sneak into the play-offs. That says enough to suggest it will be tough.
"Overall, from where we are, we've got our own agenda. We know where we are and what we've got to do to get a result up there. If we do get a good result there are a few fixtures going on that could go in our favour.
"It could be a good weekend for us but we've got to do our own business first and if anything else happens that will be a big bonus."
With the Iron currently under caretaker management, Wilson commended Daws on the job he's doing with his big chance at Glanford Park.
"He's got them well organised," said Wilson. "Nick's got a lot of experience within football clubs, albeit not at the top in the manager's position, and he's more than capable.
"You've seen Paul Heckingbottom do well at Barnsley and some of these young coaches coming through are good ones. They just need opportunities and Nick's making sure he's taking it with both hands and Scunthorpe will be very pleased with the outcome."
For Spireites all around the world, there are a number of options to follow the action in Lincolnshire.
Live commentary direct from Glandford Park will be online at PlayerHD, with Twitter the place for minute-by-minute text coverage and Facebook and Instagram also bringing you updates.