The young Spireites' good recent run came to an end on Saturday as they lost 3-1 away at Burton Albion.
Goals at the start of either half gave the Brewers the advantage and while striker George Milner halved the deficit midway through the second half Burton soon scored a decisive third and claimed the victory.
Academy manager Mark Smith assessed the match and expressed his disappointment at his side's performance.
He said: "What I saw was that on two occasions we gave them the perfect start to both halves, that gave us a mountain to climb and it was a position we shouldn't have been putting ourselves in.
"Then with 20 minutes to go we grabbed a lifeline when George Milner after some good work from Ricky German and Joe Rowley and at that point you could see them getting a bit nervy, but we shot ourselves in the foot again by conceding straight away afterwards."
Smith felt that his side looked off the pace even in the warm-up, and he will now be working hard with teh squad to prepare them for their next game.
"You felt even in the warm up that perhaps we weren't going to be at it," Smith explained. "In some respects that's how it can be with the younger players, but they need to prepare properly and make sure they are ready in all ways for the game.
"Saturday's game was in some ways the story of the season, we'd been on a short little run of form and then we get a knock back like this.
"The task is now to pick ourselves up, prepare the right way and be ready for Lincoln City away on Saturday, which promises to be another tough game."