Both Tom Anderson and Dion Donohue have Checkatrade Trophy suspensions to serve following the Spireites' Tuesday-night win at Rochdale.Centre-back Anderson was given a straight red card during the Spotland match and is now required to serve a three-game Checkatrade Trophy ban.
Full-back Donohue was issued with a a yellow card during the game - his second of the tournament, following his caution in the group-stage win over Wolverhampton Wanderers U21 - and now faces an automatic one-game suspension in the competition.
Any player picking up two yellow cards in the competition, up to and including the fourth round, will receive a one game ban.
The suspensions noted above concern the Checkatrade Trophy only, meaning that both Anderson and Donohue are free to play in upcoming Sky Bet League 1 matches.
The Spireites were drawn away to Luton in the third round of the Checkatrade Trophy earlier on Thursday, with the game to be played in early January.