The Spireites have the chance to quickly get back on the horse on Tuesday as they face another South Yorkshire rival in the shape of Doncaster Rovers at the Proact (7:45pm kick-off).
Saturday's Sheffield United loss was just the third Chesterfield defeat in 12 games, with an improved performance and result required against relegation rivals against Donny.
The Proact Stadium ticket office opens from 9am on Tuesday morning and tickets will be available to buy over the phone (01246 269300) or in person right up to kick-off, with fans also able to buy online here
and collect from the ground.
Away supporters can purchase their tickets on the day from the away ticket office, located in the away end, before the match.
Danny Wilson's men are boosted by the return from suspension of winger Jamal Campbell-Ryce, impressive since his loan arrival but sent off in the last away game at Barnsley.
The Spireites have the same squad to choose from as on Saturday, though the manager suggested on Monday that he may look to make two or three changes to the side that lost limply to Sheffield United at the weekend.
Donny 'keeper Remi Matthews is fit again and started Saturday's Wigan win, and manager Darren Ferguson has no new injury concerns ahead of the midweek match.
After their Saturday lunchtime loss, Spireites supporters would have been pleased to see relegation results largely go in their favour in the 3pm kick-offs, the club remaining four points above the drop zone with four games to go.
However, the shock result of the day came at the Keepmoat Stadium, where Doncaster - without a win in 17 games prior to kick-off - claimed a surprise 3-1 victory over title favourites Wigan Athletic to breathe fresh life into their survival hopes.
The side remain six points shy of the safety line but will feel that with similar performance levels they may still be able to pull off a great Sky Bet League 1 escape.
From the Dugout
Speaking to PlayerHD ahead of the game, Wilson was keen to stress that despite Rovers' struggles his side will not be taking their opponents lightly.
"We take nothing for granted, irrespective of where they are in the league," he said. "Psychologically you can think that because they're at the bottom they're one of the poorest sides - but they're not, far from it.
"You'll see many times that teams will upset the odds and get results that nobody expects and last week was a prime example with Doncaster. That in itself teaches a bit of a lesson, not to underestimate anybody.
"We have to make statements very early on, of course we do. We have to set our standards very high as soon as we kick off.
"I'm sure that they will also be trying to get out of the blocks really quickly so it's down to who goes and takes their chances as soon as they can."
Wilson also pointed to the important role of the home support in helping his side recover from lulls in matches and give themselves the best possible chance of success.
He explained: "We've said many times before that we need the fans, even when things aren't going in your favour that's when we really need them.
"Saturday was a let down from their point of view. We didn't really give them much to shout about, it was done and dusted after the second goal.
"We need them to be vocal and to get the support that we know they cna give us, sometimes you need a bit of a lift at time and let's hope they can do that for us."
Near or far you can follow all the action from the Proact on Tuesday night across the Spireites' official platforms.
From live commentary on PlayerHD, though the usual popular Twitter coverage to updates on Instagram and Facebook we've got every kick covered.