The Spireites have a second successive home match and will be looking to bounce straight back from last weekend's late loss after leading promotion favourites Wigan Athletic
The home fixture with Colchester United last season provided one of the year's highlights and a 6-0 win, but manager Dean Saunders will know that Tony Humes' young side are likely to pose a strong threat this time around.
After an horrendous week of injuries leading up to the Wigan match, this week the Chesterfield squad has much better news to report. In his pre-match press conference manager Dean Saunders said that quartet Sylvan Ebanks-Blake, Sam Hird, Liam O'Neil and Drew Talbot are all in with a chance of featuring on Saturday, while Lee Novak has been passed fit after receiving a dead leg last weekend.
Subject to clearance from the Football League, incoming youth loanee Jack Fitzwater is eligible for the match after arriving from West Brom. However, fellow new signing Chris Herd did not sign in time for consideration for the game.
Colchester this week bolstered their squad with the signing of striker Marvin Sordell, signed permanently after his release from Burnley - who he had played for four times already this season - at the end of the summer transfer window. The 24-year-old goes straight into the squad for the trip to the Proact.
United manager Humes also moved on Friday afternoon to solve his goalkeeper crisis, signing Preston North End stopper Jamie Jones on a loan deal, joining Elliott Parish and giving the U's two goalkeepers for Saturday's match.
The U's have also been buoyed by news that experienced pair Darren Ambrose and Chris Porter will travel with the squad, while another former Spireite, Richard Brindley, has trained all week and is fit for selection.
'Bouncebackability' has been the key theme for Saturday's hosts, and Saunders has spent the week focusing on leading much-fancied Wigan at the 80 minute mark, rather than dwelling on the final ten minutes of the match.
The match was the Spireites' second league loss of the campaign, but displayed enough positive signs to prevent Proact brows from becoming too furrowed.
Colchester make the trip to Derbyshire without a win so far this season, but have a squad mixed between hungry promising youngsters and seasoned pros with experience in higher divisions.
With fresh legs after having last weekend off due to international call-ups in their game with Sheffield United, the U's showed character in their last league game when they came from two goals down to draw 2-2 at home with Scunthorpe United.
From the Dugout
Speaking ahead of the game Saunders knows he faces a tougher task due to the injury list he contends with.
"We've had a few unlucky freak injuries," he said. "You can avoid muscle injuries to a point but we've had impact injuries like the one Lee Novak got, Sam Hird getting a cracked rib and Liam O'Neil having a crack on his knee."
The Chesterfield manager has studied and surveyed Colchester's squad and the results they've had this season and feels their current spot in the lower half of the league is not a true reflection of their capabilities.
"They're in a false position, they're a decent team," he said. "They're down the bottom of the league but they shouldn't be. They've got some good players and we know all about them, but really I'm more concerned about us and what we do - we have to keep on playing like we're playing.
"In football you get slapped in the face and you have to walk through it. We're four points off the top and a win on Saturday will get us probably into the top four."
As always, there will be full coverage of the match across the Chesterfield FC media channels. We'll have live match commentary on PlayerHD, with coverage on social media with tweets, Facebook updates and photos across the day on Instagram.