It has been confirmed that Spireites left-back Dan Jones suffered a fracture to his right ankle during the second half of Saturday's match with Swindon Town.
The injury saw the 28-year-old leave the pitch on a stretcher, and head of sports science and medicine Rodger Wylde talked through the unfortunate event.
He said: "On Saturday when I ran on to the field Dan immediately told me that he had heard something pop, which is never a good sign.
"I tested the ligaments on the ankle and found no pain there but a great deal of pain around the bone area. That made it clear to me initially that he'd not be able to continue in the match. I was suspecting a fracture, so I treated it as one.
"We took him off on a stretcher and brought him into the medical room where I re-assessed him. The specific tests I conducted showed positive signs of a fracture, so we sent him off for an X-ray."
With the injury happening in seemingly innocuous fashion and without a strong strong challenge taking place, Wylde explained how the injury had occurred.
"He was pushing and turning at the same time. You might think that couldn't cause a fracture, but whenever you have strong enough forces going through at the wrong time you can cause damage, even a fracture."
Going into further detail about the injury, Wylde explained that Jones is now likely to spend a reasonable stretch of time on the sideline.
"The X-ray confirmed a spiral fracture of the tibia, which is the inside of the ankle and he's going to see an ankle specialist tonight - the same specialist who dealt with Aaron Chapman's ankle reconstructions.
"Then we'll know how the injury should be treated and whether he'll need to be operated on.
"With a lower limb injury like this you're looking at 12 weeks for the fracture site to fuse together and possibly then another 12 weeks for consolidation, but we'll know more after seeing the specialist."