The Spireites head to Bramall Lane with their play-off place secured but energies are still guaranteed to be high with local pride at stake for the match with Sheffield United.
The two clubs are secure in fifth and sixth places in Sky Bet League 1, with the winner from Sunday's clash taking the higher position when the two sides head into the post-season.
Chesterfield still miss just defender Drew Talbot for the match through injury, though manager Paul Cook did suggest in his Friday pre-match briefing that the versatile 28-year-old does have a chance of being fit for the play-off semi-final first leg on Thursday.
Though the match is has lost some of its significance with the two sides confirming their places in the top six, Cook has said that he will be putting out a full-strength squad for the match.
Blades manager Nigel Clough has intimated, however, that three or four of his players - those carrying knocks - will miss Sunday's match in preparation for United's play-off campaign.
Absentees are likely to include Matty Done and Ryan Flynn who have been hampered this week by injuries and have eyes on fitness returns for the play-off semi-final first leg.
This could open the way for youngsters such as striker Che Adams and midfielder Louis Reed to start the match - giving them the chance to put in the sort pf performances that have earned the pair rave reviews in their debut Bramall Lane seasons.
The seven-match unbeaten march that took the Spireites into the play-offs was halted last Saturday by champions Bristol City, who impressed a packed-out Proact in beating the Blues 2-0.
However, Chesterfield can still leapfrog the Blades - who sit two points above them - and finish in fifth position in League One if they win on United's own patch this weekend.
The fifth placed-finishing side will face Swindon Town over two legs in the play-offs, while the team that ends the season in sixth will meet either MK Dons or Preston North End.
After a run of four wins out of five in late March and early April, the Blades have not won in four, drawing three of those matches and giving the Spireites the chance to spring past them on the last day of the season.
From the Dugout
Speaking to Spireites Player on Friday, Cook reported the availability of his squad and his thinking about what side to play ahead of the play-offs.
"Drew Talbot won't be involved," he said. "But we will otherwise be at full strength. I believe in continuity - there's no right way or wrong way and you just do what you think is right.
"I still think there might be a twist or turn this weekend, there always is, and all you can do is wish everyone else the best of luck. Whoever comes in the play-offs it will be a level playing field and we will all go from there."
Cook also paid tribute to the Spireites away support this season, and is looking forward to seeing the travelling troops at Bramall Lane on Sunday.
"There were spells in the season where both Nigel and myself have had up and down times, but now that we're both in the play-offs it should be a good game.
"We'll take fantastic away support," he said. "The stats I've seen about the club's away support are a great credit to Chesterfield and you must take your hat off to them.
"There have been times this season where we've come out and the hairs on your neck stand up because our supporters have travelled so well all year. We've got two more away games for sure - and hopefully one more big one after that."
For Spireites unable to get to Bramall Lane help is at hand, Sprieites Player subscribers can follow full live match commentary on Spireites Player, minute-by-minute coverage will be available on Twitter and the Spireites Wall will also provide full social media coverage.