The youth team kept their unbeaten run going, but could only draw 1-1 in a home league match against Hartlepool United on Saturday.
Striker Curtis Morrison gave the Spireites a first half lead, before the hosts were pegged back by a second half Pools strike, the points eventually shared between the sides.
Coach Mark Smith made a number of changes with the midweek Midland Floodlit Cup semi final in mind, and explained the reasoning behind the alterations.
"We've got a big cup game away against Shrewsbury on Tuesday night," said Smith. "With that in mind we decided to change the team, but even with the changes I felt we'd have more than enough to win the game.
"It wasn't a case of weakening the team, but changing it. We had enough players there with youth team experience in the league this season, but it was one of those games where we didn't start very well and never picked it up."
The youth team remain unbeaten in the league since October, and Smith was at least happy to continue the impressive streak.
He said: "It was a game we could have lost, so I'm happy that didn't happen and we keep the run going. Even though we took the lead it wasn't our best performance and I could always sense that we might concede.
"Dylan Parkin, our goalkeeper, made some good saves late on and that kept us in the game."
Chesterfield team: Parkin (gk), Holmes, Tuite, Randall, Maguire, Marshall, Jarrold, Lynam, Coy, Daly, Morrison
Subs: Fowodu, Rowley, Taylor