The Spireites will quickly have the chance to jump back on the horse after Saturday's loss, hosting Gillingham at the Proact Stadium on Tuesday night.
Individual errors saw the Spireites slump to a 3-2 weekend defeat to Coventry City, with manager Paul Cook saying after the game that such mistakes are a side-effect of his side's positive style of play.
In the reverse fixture in Kent before Christmas a depleted Spireites line-up managed to secure an unlikely 3-2 away victory and Tuesday's hosts will be desperate to record a league double and breathe fresh life into their play-off challenge.
Chesterfield have recalled full back Drew Talbot for the match and the popular defender goes straight into squad consideration for the match. However, the Spireites have also had the loan shoe on the other foot, with Sheffield Wednesday recalling striker Caolan Lavery from his spell at the Proact Stadium after Saturday's match.
Midfielder Sam Morsy misses the second match of his two-game suspension for accumulating ten yellow cards, with Ollie Banks and Ritchie Humphreys battling it out again for Morsy's central midfield spot alongside Jimmy Ryan.
The Gills have found a striker in form in on-loan Millwall man John Marquis, who has scored seven goals in 12 appearances since joining the Priestfield side in January.
Gills manager Justin Edinburgh is unlikely to be able to call on Jake Hessenthaler, who has recently been suffering with tonsillitis and missed the game at Ashton Gate on Saturday, however he will have new loan signing Ollie Muldoon at his disposal, the young midfielder on loan from Charlton Athletic, who impressed his temporary boss during his debut at the weekend.
The Spireites are in a topsy turvy run of recent form, losing four in February, before winning back-to-back against Fleetwood Town and MK Dons, but then losing at home to Coventry City on Saturday.
Despite the result the Spireites remain in the chasing pack of ten clubs with hopes of crashing May's play-off party, though Cook acknowledged in his pre-match press conference that the time to start winning is now, pin-pointing Tuesday's game as particularly crucial.
Gillingham's up and down form has been spread across the season, starting strongly, falling into a slump but then pushing forward again under the stewardship of new manager Edinburgh.
At the weekend the Gills claimed an impressive goalless draw at league-leaders Bristol City and are now just a point behind the Spireites after a three match unbeaten spurt started with the 3-0 stunning of Swindon Town at the County Ground.
From the Dugout
Speaking to Spireites Player ahead of the match, Cook explained how his squad had reacted to the weekend result and hinted at a change he might make ahead of the Gillingham game.
He said: "The lads are good, they came in yesterday (Sunday) and they were a bit down, it's my job to lift them. We've had seven and half thousand people here and a good game on Saturday, it was a game we were desperate to win and it wouldn't half have given us a fillet for the game on Tuesday.
"I won't be experimenting for the game, I think for any supporter Gboly Ariyibi looks like he could start, the lad looks lively and we've got good options."
Cook also reserved special praise for his opposite number on Tuesday, though stressed that the only focus of the match must be what his own team does in it.
"I think Justin's done a fantastic job since he's gone in," said Cook. "They were obviously down there [in the table] for whatever reason and the managers have changed.
"Justin's done great, he's a great lad and in my opinion he's one of the brighter young managers in the game. He had great success at Newport with Wembley appearances and getting Newport into the play-offs in League Two.
"He came here last year and got a draw with Newport late on and we enjoyed having a drink after the game. I look forward to seeing him tomorrow, he's done great, but it's all about us tomorrow night.
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