The Spireites head to Glanford Park on Tuesday night for their fifth, and probably final, match of the season with Scunthorpe United.
So far the league head-to-head sees the Spireites with their noses in front with two wins to the Iron's one Johnstone's Paint trophy triumph, with one further draw in the FA Cup between the sides.
There's a case of one in and one in the defensive positions for Tuesday's visitors. Sam Hird returns from his one game ban and is likely to act as a straight replacement for Ian Evatt, who suffered concussion at the weekend and misses the match.
Manager Paul Cook has said he'll make changes to the team that lost 3-2 at home to Leyton Orient on Saturday, though the game is likely to come too early for Gary Roberts, who is back in training after his hamstring injury.
Midfielder Jimmy Ryan received a dead leg at the weekend but is fit to play, as is Sam Clucas who was a doubt before the Orient match but played the full 90 minutes without problem.
Iron manager Mark Robbins will be hoping to include Isaiah Osbourne and Neal Bishop in his squad as they recover from injury, while former-Spireites striker Gary McSheffrey misses the game through suspension.
Spireites fans will be pleased not to have to face Iron goalkeeper Sam Slocombe. The stopper misses the game with a broken arm having performed outstandingly in the previous matches with Chesterfield this season.
After Saturday's defeat manager Cook was more upset with the lack of fight shown by his team than the result itself, and will be desperate to turn his side's form around and reignite the team's fight for the play-offs.
Scunthorpe's topsy-turvy season took a turn for the better with a stunning 3-1 home win over promotion chasing Swindon Town on Saturday and, despite being 11 places below Chesterfield, just five points separates the sides, illustrating the competitiveness of the middle part of the league.
Iron fans will be hoping incoming striker Kevin van Veen can continue his bright start to life at Glanford Park following the Dutchman's January move to Lincolnshire.
Cook revealed in his pre-match press conference that Van Veen had also been on the Spireites' shopping list ahead of his move to the Iron.
From the Dugout
Speaking to Spireites Player ahead of the third Spireites trip to Glanford Park this season, Cook spoke of his enjoyment when coming face to face with the Iron and their manager Mark Robbins.
"Mark's gone in and brought some new players in," said Cook. "He's a proven manager and he's been good at every club he's been at. He's a very calm character and he conducts himself really well.
"We enjoy playing Scunthorpe, they're good games. They're very competitive and we get on well with the Scunthorpe staff.
"The games have always been tough and demanding to play in, but it's a nice rivalry between the two clubs, and that will be same tomorrow night."
Cook also focused on his efforts to bring the sparkling attacking football back to his side that was missing against Orient at the weekend.
He said: "I just want us to go back to being the Chesterfield that we've all come to love, that's my big thing in football.
"The second half at Scunthorpe away [in the FA Cup] was where our football carried us over the line. Now we've just lost that a little bit and my job's to try and get us back to that."
Supporters unable to get to the match can listen to full match live commentary on Spireites Player, follow minute-by-minute text coverage on Twitter or score updates on the club's Facebook page.