One of the seasonal highlights comes early this term as the Spireites make the short journey to Sheffield United on Saturday (3pm kick-off), looking to add to their flying league start to the season.
Chesterfield last season took four points from their two Blades encounters, securing a 3-2 home win before playing out a 1-1 draw at the Lane on the last day of last season, and they'll be looking for further success during this campaign.
Friday signing Lee Novak is eligible to take part for the Spireites after completing his loan move from Birmingham City in time. Having played throughout pre-season for the Blues Novak is fit, though Dean Saunders was on Friday unwilling to give away his Saturday plans regarding his latest striking option.
In order to rest, experienced pair Sylvan Ebanks-Blake and Drew Talbot didn't travel to Carlisle United on Tuesday but are both back in contention to be in the starting 11 at Bramall Lane. However, Mani Dieseruvwe will not be involved on Saturday, starting the first of his three-game suspension after his Tuesday dismissal.
Despite the 120-minutes of action in the Capital One Cup, Saunders has said that his squad is all fit and raring to go - with the exception of striker Byron Harrison who pulled out of the warm-up at Brunton Park and faces a race to be ready for Saturday's match.
Incoming Blades manager Nigel Adkins has a large squad to choose from and with players such as Jose Baxter, Jamal Campbell-Ryce, Terry Kennedy and Craig Alcock returning to training this week, he has decisions to make before facing the Spireites.
In their Capital One Cup midweek match United made seven changes, with loan signing David Edgar impressing after making his debut, the defender also arriving from Birmingham City.
The Spireites have started the campaign with a league win and a cup loss, their opponents on Saturday starting instead with a league loss and a cup win.
Chesterfield were sent north in the Capital One Cup to Carlisle United, and despite the late Dieseruvwe leveller, the same man was soon red carded and the visiting ten men succumbed to an eventual 3-1 extra time defeat.
The Blades were shell-shocked as set piece frailties were their undoing in a 4-0 loss to Gillingham at Priestfield on the opening day of the Sky Bet League 1 season.
Their much-changed line-up restored some pride with a 1-0 win at Morecambe in the league cup, setting up a trip to the capital and Championship side Fulham in the next round.
From the Dugout
After the draining Tuesday trip to Carlisle Saunders says that the second half of the week has been spent ensuring the squad are ready to go again in the league at the weekend.
He said: "I was a bit worried about players getting injured on Tuesday or running their legs out before Saturday, because in the league we have to do well. We've started great and we have to keep that carrying on this Saturday.
"The players didn't do anything on Wednesday and they didn't do a lot on Thursday, they've just been with the fitness coach to get their legs going again and the ones who didn't play trained: they'll all be ready to play on Saturday.
Saunders - who spent two years at Bramall Lane as a player - knows well what to expect from the Spireites' South Yorkshire adversaries, but remains focused on his side and how they can win the match.
"It's a club I played for and it's a great club who will be back in the Premier League one day," Said Saunders. "We need to make sure that they don't beat us, I'm focused on Chesterfield and our team, going there and getting a result; then I wish them well after that.
"Sometimes getting a bit of a pasting in the first game helps you sort things out for the future, but I'm just focusing on what we're doing. I am aware of what they're trying to do but I don't really care; if we do what we did last Saturday we'll win."
There will be a bumper Spireites contingent at Bramall Lane, but if you're not able to be in the blue army on the day you can still follow the action. There will be live full-match commentary on Spireites PlayerHD, live minute-by-minute Twitter coverage and score updates on Facebook.