With each coming match seemingly more vital than the last, the Spireites are quickly back in action on Tuesday night, facing Colchester United at the Proact Stadium.
The match is Chesterfield's penultimate home game of the season, and the Spireites will be eyeing a three-point return to enhance their play-off hopes against a U's side entrenched in a relegation battle.
Centre back Ian Evatt is the Spireites' only injury concern - after leaving the Barnsley action before half time with a rib injury - and his availability will be assessed on Tuesday morning.
Charlie Raglan replaced him at Oakwell on Saturday and once again impressed, staking his claim to retain his spot from the start on Tuesday night.
The Spireites are otherwise fully fit for the Proact Stadium match, with manager Paul Cook promising that his side would be going all out for the win ahead of the game.
The U's boss Tony Humes will be sweating on the fitness of skipper David Fox, with the midfielder having missed the last three matches with a foot injury.
Elsewhere, Humes has a choice to make at left back between Alex Wynter and Millwall loan man Matthew Briggs, the latter having been suspended for the two games before the weekend's lost to Coventry City, when Wynter was given the nod before being replaced by Briggs in the second half.
While the Spireites were unable to add a fifth successive win at Barnsley, results elsewhere kept them four points above Rochdale - who retain their game in hand - in the League One top six, with the match with Colchester one of just four games remaining.
As much as Tuesday's hosts will be gunning for a win, Colchester will equally be desperate to pick up as many points as possible to heighten their chances of a Sky Bet League 1 escape late in the season.
The U's are three points behind Crawley Town in the final safety spot in the table and with Swindon Town and Preston North End making up their last two matches of the season United will know that they need to find form and find it quickly.
From the Dugout
Speaking to Spireites Player ahead of the fixture, Cook expressed his desire to see attacking performances for the rest of the Sky Bet League 1 season.
He said: "Going into the end of the season now against these sides we won't take a backwards step, I can rest our supporters assured on that.
"We've got to get after people, the disappoint from the weekend game was that we felt we never got after Barnsley. We'd rather get beaten getting after teams than play on our backfoot."
Despite the gap in places between the sides, Cook knows that the U's are a team that will come to the Proact to play and will offer a stern test.
"They can play, without a doubt," said Cook. "They've got a young manager who's come through there at Colchester who, in my opinion, has done a fantastic job.
"It's always harder when you're at clubs that don't have an expectancy, but we all get judged. Colchester play football the right way and they still have a really good chance of getting out of it.
"Tomorrow's a huge game for both clubs for different reasons. After the game tomorrow we'll wish them the best of luck because on their day they can hurt you, and that goes for a lot of teams in this league."
Spireites unable to make it to the Proact on Tuesday can listen to live commentary on Spireites Player, follow minute-by-minute coverage on the club's Twitter page or follow the action on the official website's Spireites Wall.