Gary Roberts' stop-start beginning to the season stalled again with the midfielder pulling out of Monday's training session after another hamstring tweak.
Roberts was substituted in the latter stages of the draw with Oldham Athletic having thought he'd received a kick to his thigh, however tests have shown that a there is a small tear in the hamstring.
The club's medical staff are continuing to assess the injury, but Roberts seems unlikely to be back in time for the trip to Walsall on Saturday.
Roberts talked through his feelings on his latest injury concern.
He said: "I'm frustrated really, it's been stop-start from day one this season. Once I came back into the side I've done alright, but I just keep picking niggling injuries up and it seems like the same one every time.
"I'm frustrated and a bit down but hopefully we'll get it sorted and I'll come back better."
Roberts didn't travel to Essex for the match with Colchester United on Tuesday night, but gave his thoughts on how the Spireites will look to bounce back from the defeat.
He said: "It's not nice to concede a late goal in any game and by all accounts we played quite well in the game. The gaffer will get them in take the positives out of the game, even though we've lost.
"We've lost by one goal again, so we're not getting battered by anyone. We've got a game again on Saturday so we need to pick ourselves up get going again."
The Spireites losses this campaign have only been by single goals, and Roberts feels this shows his team are not far away from where they want to be.
He said: "It's frustrating because you don't want to lose any game. We're not a million miles away but we're not winning as much as we'd like so we'll work hard and we'll be ready to go again come Saturday and hopefully get a win at Walsall."