The Spireites travel to local rivals Mansfield Town in the second round of the Johnstone's Paint Trophy.
Tonight will be the second time the sides have faced off in the space of ten days after having not played one another for five years.
Mansfield prevailed 1-0 in the Sky Bet League 2 game at the Proact Stadium at the end of September, ending Chesterfield's undefeated start to the season.
The Stags approach this game on the back of a 4-1 home defeat at the hands of Hartlepool United on Saturday afternoon, ending their eight-game unbeaten streak.
Chesterfield also go into this game on the back of a defeat, losing 4-3 at Morecambe on Saturday after taking a 3-0 lead into the half-time break.
As both sides received a bye in the first round of the Johnstone's Paint Trophy, this will be the teams' first fixture in this competition in the 2013/14 season.
Martin Riley returns to the squad after a three match ban.
Chris Clements will miss out on tonight's game with a knee injury.
Nathan Smith (ankle), Armand Gnanduillet (knee) and Marc Richards (thigh) are still recovering from their respective injuries and will not be available for selection.
Drew Talbot missed out on Saturday's game at Morecambe and will not make tonight's cup tie, while Ritchie Humphreys is recovering from a knock picked up in Saturday's game and will also miss out.
Gary Roberts received a straight red card in the League 2 fixture against Mansfield Town and will serve the second of his three-match ban against The Stags.
"The game tomorrow is an opportunity for me to give one or two players some game-time, so they can put themselves in the shop window for our next match."
“The game is a local derby in a cup competition, and I’m not a manager that will put out a weakened team. It’s a football match and we want to win it.”
Mansfield Town: 6/4 Draw: 12/5 Chesterfield: 17/10
Skybet have the home side as slight favourites going into Tuesday's game but believe Chesterfield have every chance of leaving with a win.
(Odds accurate as of 2pm on Tuesday, October 8)
If you can't make tonight's game you can follow the action through live commentary on Spireites Player and minute-by-minute text updates on Chesterfield's official Twitter account.
Spireites Player is also the best place for all post match reaction and highlights after the final whistle and throughout the week.
Information for Travelling Fans
Spireites fans are advised to park on the Nottingham Bound side of the Robin Hood Line at Mansfield Station.
Other useful information which you may want to use can be found here.