Southend United will visit the Proact Stadium on Saturday in the second round of the 2013/14 FA Cup.
The Spireites advanced to the second round of the competition by defeating Daventry Town 2-0 at the Proact Stadium in the first round in early November.
In last season's competition Chesterfield beat Hartlepool United 6-1 at home before losing in the second round away at Tranmere Rovers.
Chesterfield approach the fixture having lost just one game in their last nine, falling 3-2 to Newport County at Rodney Parade in the Sky Bet League 2 fixture last Sunday. The North Derbyshire side have conceded just four goals in their last eight games in all competitions.
Southend United progressed to the second round of the FA Cup by defeating Morecambe 3-0 at the Globe Arena in the first round.
The Essex side come into Saturday's game having won five of their last six games including victories over Portsmouth and Mansfield Town. However, in their last outing at Roots Hall, Southend were denied victory in the 96th minute as Cheltenham scored a penalty to secure a 1-1 draw.
The two sides have already met at the Proact Stadium this season. Armand Gnanduillet scored deep into stoppage time to award Chesterfield with a 2-1 victory in the August Sky Bet League 2 game.
Sam Morsy (knee) is still recovering from the injury picked up against Bristol Rovers and will be unavailable for Saturday. However, the midfielder is making strong progress and is likely to return earlier than expected.
Nathan Smith (ankle) is still recovering from a recurrence of his long-term problem and will miss out on the game against the Shrimpers.
Liam Cooper recevied a straight red card for a professional foul against Newport County and will serve his one-match ban against Southend on Saturday.
Will Atkinson is suspended for the FA Cup game after picking up his fifth yellow card of the season against Cheltenham Town last weekend.
Barry Corr and Freddy Eastwood have both recovered from their respective knocks and are available for Saturday.
Ryan Leonard is still unavailable through injury, while Cauley Woodrow is serving the second of his three game ban.
The Southend United boss told the club's official website: “We’ll go there on Saturday with belief. We want a good FA Cup run and we showed that in the last round when we went to Morecambe.
“The FA Cup is a special competition and I’ve had some wonderful memories. For these players it’s all about getting into the third round and drawing a big club.
“That’s the prize at the end of this game – a place in the third round – but we know we will have to be at our best to come out on top.”
Chesterfield: 1/1 Draw: 5/2 Southend United: 11/4
Skybet have Chesterfield as slight favourites going into Saturday's game but believe the away side have every chance of taking something from the match.
(Odds accurate as of 11am on Friday, December 6)
If you can't make the game on Saturday you can follow the action through live commentary on Spireites Player and minute-by-minute text updates on Chesterfield's official Twitter account.
Spireites Player is also the best place for all post match reaction and highlights after the final whistle and over the weekend.