The reserves came from behind to win 3-2 at Hull City last night.
After the home side took a 16th minute lead, Jacob Hazel equalised with a header two minutes later after getting on the end of a ball in from Mark Randall.
Hull regained the lead midway through the half from the penalty spot after Jonathan Wafula was penalised for a foul.
After Hull struck the woodwork twice, the Spireites hit back to win with two goals in the last 10 minutes.
The influential Randall played a great ball to Craig Clay whose side-foot volley was turned in by Scott Boden from six yards out.
Just a few minutes from the end, Randall’s ball from right to left found Micah Evans who scored with a fine angled shot.
John Dungworth, who was in charge of the side, was pleased with the performance.
He said: “We kept going and showed a lot of good team-spirit. The lads looked fit and strong.
“It was good to see Nathan Smith play for the full 90 minutes after being out for a few months. I thought he was outstanding.
“Mark Randall ran the show in midfield and Ben Wilson made a couple of very good saves.”
The reserves have now won four out of their five Central League games this season.
Line-up: Wilson, Wafula, Smith, Broadhead, Whittaker, Clay (Slack), Randall (Scully), Evans, Waddle, Hazel, Boden