Chesterfield will begin their defence of the Johnstone's Paint Trophy against Oldham Athletic at the Proact Stadium.
The Spireites, who clinched the trophy with a 2-0 win against Swindon Town at Wembley last season, will face the Latics in a repeat of last season's area final.
The tie will be played the week commencing Monday, September 3. The Spireites beat Oldham 2-1 at the Proact Stadium courtesy of a Danny Whitaker penalty, before Jack Lester booked John Sheridan's side a place at Wembley with a late strike at Boundary Park.
Ticket information will be announced shortly.