The youth team fell to a second consecutive EFL Youth Alliance North East defeat on Friday, losing 1-0 to Burton Albion at St George's Park, Sheffield Graves.
After a goalless first half, the Brewers scored the only goal of the game in the second period, following a set-piece.
Miguel Llera's side are next in action, when they take on Hartlepool United at St George's Park, Sheffield Graves, this Saturday (11am).
Chesterfield XI vs Burton: Jones, Howard, Hand, Sharman, Render, Bradbury, Holmes, Ofoegbu, Feurtado, Rawson, Foster.
Substitutes: Marris (gk), Turner, Russ, Emery, Walker.