Dave Bentley's young guns will be looking for a better result than the senior squad achieved on Tuesday night. It will be the second time in as many days that the Imps have travelled to Derbyshire but this time it's Tom Spall's youth side that make the journey.
Last weekend's draw against the Millers set up a nail biting clash against the Imps on Saturday with Bentley's side knowing any slip up could cost them the chance of League success.
The Imps are eighth in the North East Conference and will be full of confidence after their 2-1 victory over Doncaster Rovers last week. The last time these two sides meet, the outcome was a 1-1 draw back over in Lincolnshire but the Spireites will be looking for a much needed win.
Despite the disappointing result last weekend, the Spireites still remain unbeaten their last nine league matches.
Kick off is 11am at Warminster Road and is Chesterfield's last game at home this season.
Other fixtures in the North East Conference this Saturday:
Chesterfield v Lincoln City
Hartlepool United v Scunthorpe United (12.00)
Hull City v Grimsby Town
York City v Mansfield Town