The game against the Owls, which is on Saturday, July 27, is the fourth and final home fixture in the pre-season programme.
The Spireites also entertain Huddersfield Town, Nottingham Forest and Wolverhampton Wanderers in the build-up to the new campaign.
Details of a special ticket offer for the home pre-season fixtures will be announced at a later date.
The full list of pre-season fixtures:
Buxton (a), Saturday, July 6, kick-off 3pm.
Huddersfield Town (h), Saturday, July 13, kick-off 3pm.
Nottingham Forest (h), Tuesday, July 16, kick-off to be confirmed.
Southport (a), Saturday, July 20, kick-off 3pm.
Wolverhampton Wanderers (h), Tuesday, July 23, kick-off to be confirmed.
Sheffield Wednesday (h), Saturday, July 27, kick-off 3pm.